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primary antibodies  (Proteintech)


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    Structured Review

    Proteintech primary antibodies
    Primary Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+brca1/BRCA1+Antibody/pm41921649-56-21-25
    Average 95 stars, based on 132 article reviews
    primary antibodies - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: SERBP1 is required for efficient HR repair and cisplatin chemoresistance in lung adenocarcinoma.
    Article Snippet: The following antibodies were used in this study: anti-SERBP1 (10729-1-AP), anti-BRCA1 (22362-1-AP) (all from Proteintech, Wuhan, China), anti-Bcl-2 (#4223), anti-γ-H2AX (#9718S) (all from Cell Signaling Technology, Danvers, MA, USA), and anti-RAD51 (ab133534) (Abcam, Cambridge, UK).

    Article Title: SERBP1 is required for efficient HR repair and cisplatin chemoresistance in lung adenocarcinoma
    Article Snippet: The following antibodies were used in this study: anti-SERBP1 (10729-1-AP), anti-BRCA1 (22362-1-AP) (all from Proteintech, Wuhan, China), anti-Bcl-2 (#4223), anti-γ-H 2 AX (#9718S) (all from Cell Signaling Technology, Danvers, MA, USA), and anti-RAD51 (ab133534) (Abcam, Cambridge, UK).

    Article Title: A Novel Human Cellular System for Studying Normal Aging and for Anti‐Aging Discovery
    Article Snippet: Anti‐BRCA1 , Proteintech , 22,362–1‐AP, RRID: AB_2879090.

    Article Title: Cryptic redundancy between PAR1b and PAR1a, two members of the PAR1 kinase family, in the survival of PAR1b-knockout mice.
    Article Snippet: Anti-PAR1a/MARK3 (#9311), anti-PAR1d/MARK4 (#4834), anti-Phospho-Histone H2A.X (Ser139) (20E3) (#9718), anti-β-Actin (8H10D10) (#3700), anti-HA-Tag (C29F4) (#3724) antibodies were from Cell Signaling Technology; anti-PAR1b/MARK2 (EPR8553) and anti-PAR1a/MARK3 (EPR633Y) antibodies were from abcam; anti-PAR1c/MARK1 (21552-1-AP) antibody was from Proteintech; anti-BRCA1 (287.17), anti-BRCA1 (D-7), and anti-53BP1 (E-10) antibodies were from Santa Cruz; anti-Phospho-Histone H2A.X (Ser139) (JBW301) antibody was from Millipore.

    Article Title: Discovery of selective PARP1/CDK6 dual target inhibitors modulating Wnt signaling pathway for the treatment of TNBC.
    Article Snippet: The combination of PARP1 and CDK6 inhibitors shows synergistic anticancer effects in TNBC.. However, firstgeneration PARP inhibitors lack optimal PARP trapping efficiency and enzyme family selectivity, often leading to hematotoxicity.. Herein, we report the rational design and synthesis of a series of second-generation selective PARP1/CDK6 dual inhibitors, based on the structural features of the latest selective PARP1 inhibitor AZD5305.

    Article Title: PDGF-BB mitigates pericyte injury by activating the PHF19-PRC2 complex via the miR-221/BRCA1 signaling axis in Alzheimer's disease.
    Article Snippet: The antibodies used included anti-PDGF-BB, anti-BRCA1, mouse anti-EZH2, anti-EED, anti-SUZ12, anti-α-SMA, anti-Desmin, anti-GAPDH, and anti-IgG-HRP, all obtained from Abcam, except for anti-RbAp46/48 (Cell Signaling Technology), anti-PHF19 (Proteintech), and anti-PDGFRβ (Proteintech).

    Article Title: Cryptic redundancy between PAR1b and PAR1a, two members of the PAR1 kinase family, in the survival of PAR1b -knockout mice
    Article Snippet: Anti-PAR1a/MARK3 (#9311), anti-PAR1d/MARK4 (#4834), anti-Phospho-Histone H2A.X (Ser139) (20E3) (#9718), anti-β-Actin (8H10D10) (#3700), anti-HA-Tag (C29F4) (#3724) antibodies were from Cell Signaling Technology; anti-PAR1b/MARK2 (EPR8553) and anti-PAR1a/MARK3 (EPR633Y) antibodies were from abcam; anti-PAR1c/MARK1 (21552-1-AP) antibody was from Proteintech; anti-BRCA1 (287.17), anti-BRCA1 (D-7), and anti-53BP1 (E-10) antibodies were from Santa Cruz; anti-Phospho-Histone H2A.X (Ser139) (JBW301) antibody was from Millipore.

    Western Blot:

    Article Title: Development of the first-in-class RNF4 PROTAC degrader as potential therapeutics for hepatocellular carcinoma.
    Article Snippet: Ring Finger Protein 4 (RNF4) recognizes poly-SUMOylated proteins via its SUMO-Interacting Motifs (SIMs) and subsequently ubiquitinates them, thus effecting some key regulatory proteins involved in cancer development and progression.. Our previous study found RNF4 was a potential target for HCC interruption.. However, none of its inhibitor has been developed so far.



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    A . MCF7 cells were treated for 1 h with DMSO or 50 μM PUGNAc ± 10 μM veliparib, irradiated with 6 Gy, fixed after 2 h and stained for 53BP1 and <t>BRCA1.</t> Representative images with 53BP1 foci (green), BRCA1 foci (red), and DAPI (blue) are shown. B , C . Plots of 53BP1 ( B ) and BRCA1 ( C ) foci per nucleus at 2 h after 6 Gy in DAPI Low and DAPI High cells. D . Cells were treated as in A but fixed after 24 h. E, F . Plots of 53BP1 ( E ) and BRCA1 ( F ) foci per nucleus at 24 h after 6 Gy in DAPI Low and DAPI High cells. For images, scale bar = 20 μm; inset is mean ± SEM foci per nucleus. For plots, red bars indicate mean ± SEM; ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05 (unpaired t-test).
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    Image Search Results


    A . MCF7 cells were treated for 1 h with DMSO or 50 μM PUGNAc ± 10 μM veliparib, irradiated with 6 Gy, fixed after 2 h and stained for 53BP1 and BRCA1. Representative images with 53BP1 foci (green), BRCA1 foci (red), and DAPI (blue) are shown. B , C . Plots of 53BP1 ( B ) and BRCA1 ( C ) foci per nucleus at 2 h after 6 Gy in DAPI Low and DAPI High cells. D . Cells were treated as in A but fixed after 24 h. E, F . Plots of 53BP1 ( E ) and BRCA1 ( F ) foci per nucleus at 24 h after 6 Gy in DAPI Low and DAPI High cells. For images, scale bar = 20 μm; inset is mean ± SEM foci per nucleus. For plots, red bars indicate mean ± SEM; ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05 (unpaired t-test).

    Journal: Journal of cell science

    Article Title: Cancer metabolism in radiation sensitization: complementary roles of O-GlcNAc transferase (OGT) and PARP1

    doi: 10.1242/jcs.264322

    Figure Lengend Snippet: A . MCF7 cells were treated for 1 h with DMSO or 50 μM PUGNAc ± 10 μM veliparib, irradiated with 6 Gy, fixed after 2 h and stained for 53BP1 and BRCA1. Representative images with 53BP1 foci (green), BRCA1 foci (red), and DAPI (blue) are shown. B , C . Plots of 53BP1 ( B ) and BRCA1 ( C ) foci per nucleus at 2 h after 6 Gy in DAPI Low and DAPI High cells. D . Cells were treated as in A but fixed after 24 h. E, F . Plots of 53BP1 ( E ) and BRCA1 ( F ) foci per nucleus at 24 h after 6 Gy in DAPI Low and DAPI High cells. For images, scale bar = 20 μm; inset is mean ± SEM foci per nucleus. For plots, red bars indicate mean ± SEM; ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05 (unpaired t-test).

    Article Snippet: The primary antibodies used were 53BP1, BRCA1 (Santa Cruz Biotechnology; sc-6954; 1:1,000), and RAD51 (Novus Biologicals; NB100–148; 1:1,000).

    Techniques: Irradiation, Staining

    A . MCF7 cells were incubated with 10 μM BrdU for 24 h, then treated for 1 h before 6 Gy with DMSO ± 10 μM veliparib or 50 μM PUGNAc + 10 μM veliparib, fixed after 24 h and single strand DNA detected with anti-BrdU antibody under non-denaturing conditions. Representative images show BrdU foci in red with DAPI in blue. B-D . Cells were treated for 1 h before 6 Gy with DMSO ± 10 μM veliparib or 50 μM PUGNAc + 10 μM veliparib, fixed after 24 h and probed for RPA ( B ), RAD51 ( C ) or BRCA1 ( D ). E-H . Plots of BrdU ( E ), RPA ( F ), RAD51 ( G ), and BRCA1 ( H ) foci per nucleus in at 24 h after 6 Gy. For images, scale bar = 20 μm; inset is mean ± SEM foci per nucleus. For plots, red bars indicate mean ± SEM; ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05 (unpaired t-test).

    Journal: Journal of cell science

    Article Title: Cancer metabolism in radiation sensitization: complementary roles of O-GlcNAc transferase (OGT) and PARP1

    doi: 10.1242/jcs.264322

    Figure Lengend Snippet: A . MCF7 cells were incubated with 10 μM BrdU for 24 h, then treated for 1 h before 6 Gy with DMSO ± 10 μM veliparib or 50 μM PUGNAc + 10 μM veliparib, fixed after 24 h and single strand DNA detected with anti-BrdU antibody under non-denaturing conditions. Representative images show BrdU foci in red with DAPI in blue. B-D . Cells were treated for 1 h before 6 Gy with DMSO ± 10 μM veliparib or 50 μM PUGNAc + 10 μM veliparib, fixed after 24 h and probed for RPA ( B ), RAD51 ( C ) or BRCA1 ( D ). E-H . Plots of BrdU ( E ), RPA ( F ), RAD51 ( G ), and BRCA1 ( H ) foci per nucleus in at 24 h after 6 Gy. For images, scale bar = 20 μm; inset is mean ± SEM foci per nucleus. For plots, red bars indicate mean ± SEM; ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05 (unpaired t-test).

    Article Snippet: The primary antibodies used were 53BP1, BRCA1 (Santa Cruz Biotechnology; sc-6954; 1:1,000), and RAD51 (Novus Biologicals; NB100–148; 1:1,000).

    Techniques: Incubation

    A, B . shScr, shOGT, and shOGA were induced for 48 h with 1 μg/ml doxycycline and cells were treated with 0 or 10 μM veliparib for 1 h prior to 6 Gy, fixed after 24 h and stained for BRCA1. Shown are representative images ( A ) and plots of BRCA1 foci per nucleus ( B ). C, D . Cells were treated as in A and stained for RPA. Shown are representative images ( C ) and plots of RPA foci per nucleus ( D ). E . shScr, shOGT, and shOGA cells were treated as in A and then lightly permeabilized, fixed and then stained with anti-DNA to detect cytosolic DNA (cytoDNA). Shown are representative pseudo-colored images, with cytoDNA in red, DAPI counterstain in blue, and overlays. For images, scale bar = 20 μm; inset is mean ± SEM foci per nucleus. For plots, red bars indicate mean ± SEM; ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05 (unpaired t-test).

    Journal: Journal of cell science

    Article Title: Cancer metabolism in radiation sensitization: complementary roles of O-GlcNAc transferase (OGT) and PARP1

    doi: 10.1242/jcs.264322

    Figure Lengend Snippet: A, B . shScr, shOGT, and shOGA were induced for 48 h with 1 μg/ml doxycycline and cells were treated with 0 or 10 μM veliparib for 1 h prior to 6 Gy, fixed after 24 h and stained for BRCA1. Shown are representative images ( A ) and plots of BRCA1 foci per nucleus ( B ). C, D . Cells were treated as in A and stained for RPA. Shown are representative images ( C ) and plots of RPA foci per nucleus ( D ). E . shScr, shOGT, and shOGA cells were treated as in A and then lightly permeabilized, fixed and then stained with anti-DNA to detect cytosolic DNA (cytoDNA). Shown are representative pseudo-colored images, with cytoDNA in red, DAPI counterstain in blue, and overlays. For images, scale bar = 20 μm; inset is mean ± SEM foci per nucleus. For plots, red bars indicate mean ± SEM; ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05 (unpaired t-test).

    Article Snippet: The primary antibodies used were 53BP1, BRCA1 (Santa Cruz Biotechnology; sc-6954; 1:1,000), and RAD51 (Novus Biologicals; NB100–148; 1:1,000).

    Techniques: Staining